In this article, we will use the Pathways Tool to investigate the pathways related to gene-expression patterns in a Next-Generation Clustered Heat Map (NG-CHM). Specifically, we will explore our TCGA THYM rnaSEQ gene by sample map. The samples in this map have been grouped into four clusters based on hierarchical gene-expression clustering, as shown in the top covariate bar.

In this article, we will create a Next-Generation Clustered Heat Map (NG-CHM) of the 200 most variable microRNAs in the TCGA prostate cancer data available from the ISB Cancer Genomics Cloud (ISB-CGC).

For creating NG-CHMs we have a variety of tools for different usage scenarios: